The Gastrointestinal Microbiome in Infant Colic: A Scoping Review.

ABSTRACT: The significant crying of infantile colic adds stress to the infant and their family, yet it has no recognized etiology. Gastrointestinal health problems and dysfunction have been suspected in the etiology of colic. Disruptions to the microbiome colonization of the gastrointestinal system may lead to excess gas and inflammation that are associated with the crying of colic. Infants with colic have increased colonization with gas-producing bacteria, like Escherichia coli and Klebsiella , and they have lower colonization of anti-inflammatory bacteria, like Bifidobacterium and Lactobacillus . Colic is known to self-resolve around 3 months of age. However, few researchers have investigated how the microbiome may be changing at colic's natural resolution without the intervention of a probiotic. With a better understanding of what leads to colic's self-resolution, future researchers may be able to identify more effective therapies for colic prevention or treatment. This scoping review presents the collective evidence from 21 original, primary research articles on what is known about the gastrointestinal microbiome at colic onset and resolution.

Brain Science-Based Innovative Collaborative Teaching to Facilitate Nursing Education.

BACKGROUND: Innovative teaching that effectively promotes learning is a process called brain science. Chemicals released during motivation and attention lead to improved learning, and chemicals released during high-stress situations deter learning. The coronavirus disease 2019 (COVID-19) pandemic has created unprecedented stress while providing an opportunity to create innovative strategies for facilitated learning. METHOD: To meet the pandemic challenges of a traditional undergraduate nursing program at a large state-funded university, specialty course faculty collaborated to redesign the courses using brain science concepts. RESULTS: Students demonstrated improved average course scores across courses compared with previous students (obstetrics, 2%; pediatrics, 4.34%; and critical care, 1.38%). Overall student feedback was positive. CONCLUSION: Brain science provides the foundation for advanced education that promotes optimal learning. The stress of the COVID-19 pandemic has created the opportunity for the implementation of an advanced educational model in which learning is facilitated and supported. [J Nurs Educ. 2022;61(3):162-166.].

Promoting and Protecting the Gastrointestinal Newborn Microbiome Through Breastfeeding Practices.

Since newborns are a vulnerable population that cannot protect their own microbiome, healthcare professionals can promote, advocate, and assist with breastfeeding promotion to protect the healthy development of the newborn gastrointestinal microbiome. The newborn gastrointestinal microbiome is a dynamic community of bacteria that influence health. Breastfeeding seeds and feeds the newborn gastrointestinal microbiome. A disruption in the balance of the gastrointestinal microbiome can result in adverse health outcomes. This clinical article makes an evidence-based connection between breastfeeding and the establishment of the newborn gastrointestinal microbiome through breastfeeding promotion strategies during the childbearing year. Suggestions for healthcare profession education and future research that will continue to inform the understanding of healthy development of the microbiome will be provided. By assisting with breastfeeding promotion, healthcare professionals can protect the newborn gastrointestinal microbiome and promote overall newborn, infant, and child health.

Pyrosequencing-based validation of a simple cell-suspension polymerase chain reaction assay for Campylobacter with application of high-processivity polymerase and novel internal amplification controls for rapid and specific detection.

Although Campylobacter is an important food-borne human pathogen, there remains a lack of molecular diagnostic assays that are simple to use, cost-effective, and provide rapid results in research, clinical, or regulatory laboratories. Of the numerous Campylobacter assays that do exist, to our knowledge none has been empirically tested for specificity using high-throughput sequencing. Here we demonstrate the power of next-generation sequencing to determine the specificity of a widely cited Campylobacter-specific polymerase chain reaction (PCR) assay and describe a rapid method for direct cell suspension PCR to quickly and easily screen samples for Campylobacter. We present a specific protocol which eliminates the need for time-consuming and expensive genomic DNA extractions and, using a high-processivity polymerase, demonstrate conclusive screening of samples in <1 h. Pyrosequencing results show the assay to be extremely (>99%) sensitive, and spike-back experiments demonstrated a detection threshold of <10(2) CFU mL(-1). Additionally, we present 2 newly designed broad-range bacterial primer sets targeting the 23S rRNA gene that have wide applicability as internal amplification controls. Empirical testing of putative taxon-specific assays using high-throughput sequencing is an important validation step that is now financially feasible for research, regulatory, or clinical applications.